Budapest Biostruct Course on Basics in Protein Crystallization and Crystallography 2013

Day 1, August 30 Friday


Arrival-registration

Speakers' Dinner


Day 2, August 31, Saturday



9:15-9:30 Welcome and opening remarks (Beáta Vértessy, Budapest Univ. Technology and Hung. Acad. Sci, Budapest, Hungary)

09:30-10.30 Lecture 1: Structural biology with synchrotron radiation and free electron lasers (Matthias Wilmanns, EMBL Hamburg Outstation, Hamburg, Germany)

10:30-10:50 Coffee break

10:50- 12:30 Students presentations 10 mins (5+5)


12:30-14:00 Lunch


14:00-16:00 Students presentations 10 mins (5+5)

16:00-16:30 Coffee break

16:30 Tour of the Biostruct Laboratory

Walking tours , ruin pub (romkocsma)



Day 3 September 1, Sunday


9:00-10:30 Lectures in crystallization 1: From protein solution to protein crystal (Bernhard Rupp, k. k. Hofkristallamt, USA; visiting Prof. at Medical University Innsbruck, Austria)

10:30-11:00 Coffee break

11:00-12:30 Lectures in crystallization 2: Growing, optimizing and handling of protein crystal (Bernhard Rupp, k. k. Hofkristallamt, USA; visiting Prof. at Medical University Innsbruck, Austria)


12:30-14:00 Lunch


14:00-15:30 Practical 1/2/3 : 

                           Setup of drops 

                           Crystal observation

                           Seeding and crystal handling 

 (Ibolya Leveles, Veronika Harmat, Beáta Vértessy, Kinga Nyíri, Klaudia Kovács, László Radnai, Gergely Nagy, Krisztian Fodor, Ábris Bendes, Imre Zagyva)

15:30-16:00 coffee break

16:00-17:30 Practical 1/2/3 :

                           Setup of drops

                           Crystal observation

                           Seeding and crystal handling 

(Ibolya Leveles, Veronika Harmat, Beáta Vértessy, Kinga Nyíri, Klaudia Kovács, László Radnai, Gergely Nagy, Krisztian Fodor, Ábris Bendes, Imre Zagyva)

17:30-18:00 Q&A (box)

Free evening



Day 4 September 2, Monday


9:30-11:00 Visit to the Protein Crystallography Laboratory of Eötvös Lóránd University (Budapest, Pázmány Péter sétány 1/A)

Protein crystallography lab.


11:00-11:30 Coffee break

11:30-13:00 Practical 1/2/3 :

                           Setup of drops

                           Crystal observation

                           Seeding and crystal handling 

(Ibolya Leveles, Veronika Harmat, Beáta Vértessy, Kinga Nyíri, Klaudia Kovács, László Radnai, Gergely Nagy, Krisztian Fodor, Ábris Bendes, Imre Zagyva)


13:00-14:00 Lunch


14:00-15:30 Basic lectures in crystallography (Veronika Harmat, Eotvos Lorand University (ELTE) , Budapest, Hungary)

15:30-16:00 Coffee break

16:00-17:30 Data collection (Manfred Weiss, Helmholtz-Zentrum Berlin für Materialien und Energie (HZB), Berlin, Germany)

Free evening



Day 5, September 3, Tuesday 


9:00-10:00 Data processing (Veronika Harmat, Eotvos Lorand University (ELTE) , Budapest, Hungary)

10:00-11:30 Solving the phase problem (phase determination) (Manfred Weiss, Helmholtz-Zentrum Berlin für Materialien und Energie (HZB), Berlin, Germany)

11:30-12:00 Coffee break

12:00-13:30 Model building, refinement and validation (Veronika Harmat and Krisztián Fodor, Eotvos Lorand University (ELTE) , Budapest, Hungary)


13:30-14:30 Lunch


14:30 -16:00 Practical 4/5:

                           Crystal mounting and data collection 

                           Data processing and model building

(Zoltán Gál, Ibolya Leveles, Veronika Harmat, Kinga Nyíri, Gergely Nagy)

16:00-16:30 Coffee break

16:30-18:00 Practical 4/5: 

                           Crystal mounting and data collection 

                           Data processing and model building

(Zoltán Gál, Ibolya Leveles, Veronika Harmat, Kinga Nyíri, Gergely Nagy)

18:00-18:30 How to publish crystallographic results? (Manfred Weiss, Helmholtz-Zentrum Berlin für Materialien und Energie (HZB), Berlin, Germany) 

Followed by discussions on individual cases, how to apply for a synchrotron trip, Q and A (Manfred Weiss, Veronika Harmat, Zoltán Gál, Krisztián Fodor, all lecturers and tutors)

19:00 Closing remarks (Beáta Vértessy, Budapest Univ. Technology and Hung. Acad. Sci, Budapest, Hungary)


19:30 Dinner